Presentation by: Tyler Deutsch, Mary Ruth Shifflett, and Dr. Barnabas Otoo
Abstract: ATP is a ubiquitous biological molecule known to be relatively stable in basic conditions. The pKa’s of its three acidic protons have been computed to be 6.6, 3.0 and less than 0. To better understand variant pH effects of ATP’s stability, 5 mM solutions of ATP at pH ranging from 2 to 12 were prepared. Aliquots of these solutions were analyzed via UV-Vis spectroscopy. The results show a deviation from the normal absorption plot at pH less than 5. Validating that ATP decomposes under acidic conditions.
Thank you for sharing your work!
Really nice work, Tyler, Mary Ruth, and Dr. Otoo. ATP is such an important molecule in cells and reagent in laboratories that stability studies like this are sorely needed.